We seek to bridge the gap between scientific knowledge and implementation for translation, including diagnosis, prognosis and treatment of relevant diseases in Colombia (dengue and cancer). To do this, we used tools from molecular cell biology, virology, network and data science, organic chemistry, and computational biology. By definition, translational medicine attempts to translate findings from the laboratory to the bedside of the patient.
Cancer Research Center and Institute of Molecular and Cellular Biology of Cancer.
Spanish National Research Council.
Universidad de Salamanca, Spain.
Institute of Ecology, Universidad Autónoma Nacional de México.
Department of Infectious and Parasitic Diseases FM, University of São Paulo, Brazil.
Molecular Immunology Group, Universidad del Quindío.
Theoretical and Computational RNomics Group, Universidad Nacional de Colombia, Bogotá Campus.
Ecology and Biogeography Research Group, Universidad de Pamplona, Santander.
Episteme: Philosophy and Science Group, Universidad del Valle.
Universidad Santo Tomás de Aquino, Bogotá Campus.
National Center for Biotechnology, Madrid, Spain.
DENGUE: endothelial dysfunction, a new turn for the urgent need for the pathophysiological approach to understand the disease.
Molecular pathology of glioblastoma multiforme. Chemotherapeutic resistance mechanisms tests based on miRNAs.
Non-coding RNAs: possible biomarkers of dengue progression, a new strategy in molecular and translational medicine against dengue.
Assessment of the antiviral properties of artificial miRNAs directed against cellular genes in DENV infections.
Cellular and molecular targets with useful potential in therapy.
Evolutionary ecology of emerging viruses.
Main Research Results
We have studied the phylogenetic history of dengue (DENV) in Colombia, finding the existence of a new Asian-American genotype. In addition, we have discovered several promising antiviral candidates for dengue (lovastatin, ferruginol derivates, and others), as well as methods for molecular diagnoses based on microRNAs, both for dengue and glioblastoma. Researching DENV, we have discovered that the virus uses a repertoire of cellular and molecular components common to cancer. Therefore, we do not aim towards non-conventional antiviral drugs (addressed directly to viruses), but rather the ones addressed against host targets (such as signaling, cytoskeletal elements, and vesicle trafficking).
Confocal and advanced fluorescence microscopy:
Preliminary study of the question posed by the researcher in order to determine if the answer can be obtained using confocal or advanced fluorescence microscopy, or if a different methodology is needed.
Advice on experimental design and preparation of the sample for its proper assembly.
Rental and advice for the handling of: Real-time thermal cycler (CFX-96 Bio-Rad) for different research purposes. It can quantify mRNAs, microRNAs, and various mutations for molecular pathology.
NanoDrop spectrophotometer (total spectrum: 220-750nm), used to measure concentrations of nucleic acids and proteins with a 1 µl sample, among others.